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flowmax cytometer pasiii  (Partec)
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Partec flowmax cytometer pasiii
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax Cytometer Pasiii, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowmax 3.0 flow cytometer  (Partec)
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Partec flowmax 3.0 flow cytometer
Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow <t>cytometry</t> during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.
Flowmax 3.0 Flow Cytometer, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flowmax 3.0 flow cytometer/product/Partec
Average 90 stars, based on 1 article reviews
flowmax 3.0 flow cytometer - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

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Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow cytometry during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.

Journal: Immunology

Article Title: Interleukin‐6 signalling mediates Galectin‐8 co‐stimulatory activity of antigen‐specific CD 4 T‐cell response

doi: 10.1111/imm.12980

Figure Lengend Snippet: Antigen‐presenting cells (APC) produce interleukin‐6 (IL‐6) during galectin‐8 (Gal‐8) ‐induced co‐stimulation. IL‐6 intracellular expression was determined by flow cytometry during Gal‐8‐induced co‐stimulation. Splenocytes (4 × 106) from DO11.10 mice were cultured for 24 hr in the presence of 0·1 μg/ml of ovalbumin323–339 (OVA 323–339) peptide and 0·2 μm of Gal‐8 (OVA + Gal‐8), or left untreated (Control). Cells were labelled with specific monoclonal antibodies for surface antigens (MHCII, CD11b, CD11c, F4/80, CD4 and B220) and for intracellular IL‐6. IL‐6+ population is box enfolded in the counter plots. Bars indicate percentage of IL‐6+ cells and the mean fluorescence intensity (MFI). FSC, forward side scatter. Depicted assays are representative of two independent experiments and were carried out, each time, with different recombinant protein preparations. *P < 0·05; **P < 0·01; ***P < 0·001.

Article Snippet: Flow cytometry analysis FlowMax cytometer PASIII (Partec, Münster, Germany) and flowjo software (FlowJo, Ashland, OR, USA) were used throughout this work.

Techniques: Expressing, Flow Cytometry, Cell Culture, Fluorescence, Recombinant